Journal: Journal of visualized experiments : JoVE

Article Title: Direct-Coupled Electroretinogram (DC-ERG) for Recording the Light-Evoked Electrical Responses of the Mouse Retinal Pigment Epithelium

doi: 10.3791/61491

Figure Lengend Snippet: Table of Materials

Article Snippet: Maintain at RT In-Line Filter Whatman 6722–5001 To protect vacuum pump from aerosols Low Noise Cable for Microelectrode Holders WPI Inc 5372 Suggested for improving the length and placement of the cables and electrode holder assemblies Magnetic Ball Joint WPI Inc 500871 For magnetically positioning the electrode holder assembly on the stage MatLab Mathworks MatLab: For editing the analysis software MatLab Curvefit Toolbox Mathworks Toolbox for MatLab (only required for editing the analysis software) MatLab Compiler Mathworks Toolbox for MatLab (only required for editing and re-releasing the analysis software) MatLab Runtime version 9.5 Mathworks R2018b (9.5) Required to run the analysis software: https://www.mathworks.com/products/compiler/matlab-runtime.html Microelectrode Holders (45 degrees) WPI Inc MEH345–15 For holding the capillaries Needle (25 ga) Covidien 8881250313 For filling the capillary tubes with HBSS Needle (ground) Electrode Rhythmlink 13mm - one elctrode Subdermal needle electrode (ground) for mouse (13mm long, 0.4mm diameter needle, 1.5m leadwire) Regulator/Power Conditioner Furman P-1800 Or equivalent to remove DC-offset from noise introduced through power line Syringe (12 mL) Monoject 1181200777 For filling the capillary tubes with HBSS T-clip Cole-Parmer 06852–20 For electrode holder assembly Vacuum Desiccator Bel-Art 420120000 Clear polycarbonate bottom & cover Pharmacological treatment Lubricant eye gel Alcon 0078–0429-47 Helps lubricate corneal surface and maintain electrical contact with capillary electrodes Phenylephrine Hydrochloride 2.5% Akorn 17478–201-15 Short acting mydriatic eye drops (for pupil dilation) Proparacaine Hydrochloride 0.5% Akorn 17478–263-12 Local anesthetic for ophthalmic instillation Tropicamide 0.5% Akorn 17478–101-12 Short acting mydriatic eye drops (for pupil dilation) Xylazine AnaSed sc-362949Rx Analgesic and muscle relaxant Zetamine (Ketamine HCl) VetOne 501072 Anesthetic for intramuscular injections Open in a separate window list-behavior=simple prefix-word= mark-type=none max-label-size=4 1.1.

Techniques: Convection, Software